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Depositordc.contributorArlt, J
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Funderdc.contributor.otherERC - European Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Data Creatordc.creatorSchwarz-Linek, Jana
Data Creatordc.creatorArlt, Jochen
Data Creatordc.creatorJepson, Alys
Data Creatordc.creatorDawson, Angela
Data Creatordc.creatorVissers, Teun
Data Creatordc.creatorMiroli, Dario
Data Creatordc.creatorPilizota, Teuta
Data Creatordc.creatorMartinez, Vincent A
Data Creatordc.creatorPoon, Wilson CK
Date Accessioneddc.date.accessioned2015-08-14T11:11:01Z
Date Availabledc.date.available2015-08-14T11:11:01Z
Citationdc.identifier.citationSchwarz-Linek, Jana; Arlt, Jochen; Jepson, Alys; Dawson, Angela; Vissers, Teun; Miroli, Dario; Pilizota, Teuta; Martinez, Vincent A; Poon, Wilson CK. (2015). Escherichia coli as a model active colloid: A practical introduction, [dataset]. University of Edinburgh. https://doi.org/10.7488/ds/290.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/839
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/290
Dataset Description (abstract)dc.description.abstractThe flagellated bacterium Escherichia coli is increasingly used experimentally as a self-propelled swimmer. To obtain meaningful, quantitative results that are comparable between different laboratories, reproducible protocols are needed to control, ‘tune’ and monitor the swimming behaviour of these motile cells. We critically review the knowledge needed to do so, explain methods for characterising the colloidal and motile properties of E. coli cells, and propose a protocol for keeping them swimming at constant speed at finite bulk concentrations. In the process of establishing this protocol, we use motility as a high-throughput probe of aspects of cellular physiology via the coupling between swimming speed and the proton motive force. This dataset contains the raw data to go with the published manuscript, including the movie to go with figure 1. N.B. the TIF file itself contains most of the relevant metadata, e.g. pixel calibration and frame rate etc.en_UK
Dataset Description (TOC)dc.description.tableofcontentsSee Readme.txten_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburghen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1016/j.colsurfb.2015.07.048en_UK
Relation (Is Referenced By)dc.relation.isreferencedbySchwarz-Linek et al, "Escherichia coli as a model active colloid: a practical introduction", Colloids and Surfaces B: Biointerfaces. doi:10.1016/j.colsurfb.2015.07.048.
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectExperimental protocolsen_UK
Subjectdc.subjectEscherichia colien_UK
Subjectdc.subjectCell motilityen_UK
Subject Classificationdc.subject.classificationBiological Sciences::Applied Molecular Biology Biophysics and Biochemistryen_UK
Titledc.titleEscherichia coli as a model active colloid: A practical introductionen_UK
Typedc.typedataseten_UK

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