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Depositordc.contributorAndrew, Theo
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Data Creatordc.creatorSuslak, Thomas J
Data Creatordc.creatorWatson, Sonia
Data Creatordc.creatorThompson, Karen J
Data Creatordc.creatorShenton, Fiona C
Data Creatordc.creatorBewick, Guy S
Data Creatordc.creatorArmstrong, J Douglas
Data Creatordc.creatorJarman, Andrew P
Date Accessioneddc.date.accessioned2015-06-08T09:52:44Z
Date Availabledc.date.available2015-06-08T09:52:44Z
Citationdc.identifier.citationSuslak, Thomas J; Watson, Sonia; Thompson, Karen J; Shenton, Fiona C; Bewick, Guy S; Armstrong, J Douglas; Jarman, Andrew P. (2015). Piezo is essential for amiloride-sensitive stretch-activated mechanotransduction in larval Drosophila dorsal bipolar dendritic sensory neurons., [dataset]. https://doi.org/10.7488/ds/255.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/788
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/255
Dataset Description (abstract)dc.description.abstractStretch-activated afferent neurons, such as those of mammalian muscle spindles, are essential for proprioception and motor co-ordination, but the underlying mechanisms of mechanotransduction are poorly understood. The dorsal bipolar dendritic (dbd) sensory neurons are putative stretch receptors in the Drosophila larval body wall. We have developed an in vivo protocol to obtain receptor potential recordings from intact dbd neurons in response to stretch. Receptor potential changes in dbd neurons in response to stretch showed a complex, dynamic profile with similar characteristics to those previously observed for mammalian muscle spindles. These profiles were reproduced by a general in silico model of stretch-activated neurons. This in silico model predicts an essential role for a mechanosensory cation channel (MSC) in all aspects of receptor potential generation. Using pharmacological and genetic techniques, we identified the mechanosensory channel, DmPiezo, in this functional role in dbd neurons, with TRPA1 playing a subsidiary role. We also show that rat muscle spindles exhibit a ruthenium red-sensitive current, but found no expression evidence to suggest that this corresponds to Piezo activity. In summary, we show that the dbd neuron is a stretch receptor and demonstrate that this neuron is a tractable model for investigating mechanisms of mechanotransduction. The files comprise the raw data that are the basis of our work "Piezo is essential for amiloride-sensitive stretch-activated mechanotransduction in larval Drosophila dorsal bipolar dendritic sensory neurons." [PLoS One, DOI:10.1371/journal.pone.0130969]. "Data" contains results from our Drosophila work, while "Rat_data" contains the mammalian electrophysiology.en_UK
Languagedc.language.isoengen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1371/journal.pone.0130969en_UK
Relation (Is Referenced By)dc.relation.isreferencedby"Piezo is essential for amiloride-sensitive stretch-activated mechanotransduction in larval Drosophila dorsal bipolar dendritic sensory neurons." PLoS One, DOI:10.1371/journal.pone.0130969
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectDrosophilaen_UK
Subjectdc.subjectPiezoen_UK
Subjectdc.subjectmechanosensoryen_UK
Subject Classificationdc.subject.classificationBiological Sciences::Cell Biologyen_UK
Titledc.titlePiezo is essential for amiloride-sensitive stretch-activated mechanotransduction in larval Drosophila dorsal bipolar dendritic sensory neurons.en_UK
Typedc.typedataseten_UK

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