Sargison, Fiona; Alves, Joana; Pickering, Amy C; Fitzgerald, J Ross. (2020). Staphylococcus aureus secreted lipases do not inhibit innate immune killing mechanisms: Extended Figure 1, [dataset]. University of Edinburgh. College of Medicine and Veterinary Medicine. Roslin Institute. Infection and Immunity. https://doi.org/10.7488/ds/2881.
Staphylococcus aureus pathogenesis is mediated by a plethora of secreted proteins, many of which remain incompletely characterised. For example, S. aureus abundantly secretes two isoforms of the enzyme lipase into the extracellular milieu, where they scavenge upon polymeric triglycerides. To analyse the role of lipases in host-pathogen interactions, recombinant S. aureus lipase 1 and 2 were purified from a ClearColi® BL21 (DE3). To confirm recombinant lipase 1 and 2 were functional proteins, a turbiometric assay was employed. Functional recombinant lipases were used in our studies to help broaden our understanding of the interaction of S. aureus lipases with the innate immune system.
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