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Depositordc.contributorOi, Curran
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherNIH - National Institutes of Healthen_UK
Funderdc.contributor.otherUniversity of Edinburghen_UK
Funderdc.contributor.otherThe Euan MacDonald Centreen_UK
Funderdc.contributor.otherUK Dementia Research Institute
Funderdc.contributor.otherDr. Jim Love and UCB Pharma (funding for the microscope)
Data Creatordc.creatorOi, Curran
Data Creatordc.creatorHorrocks, Mathew
Data Creatordc.creatorGidden, Zoe
Data Creatordc.creatorRegan, Lynne
Date Accessioneddc.date.accessioned2020-06-29T15:55:05Z
Date Availabledc.date.available2020-06-29T15:55:05Z
Citationdc.identifier.citationOi, Curran; Horrocks, Mathew; Gidden, Zoe; Regan, Lynne. (2020). LIVE-PAINT Supporting Datasets, [dataset]. University of Edinburgh. Quantitative Biology, Biochemistry and Biotechnology. https://doi.org/10.7488/ds/2859.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3678
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2859
Dataset Description (abstract)dc.description.abstractWe present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extend data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein.en_UK
Dataset Description (TOC)dc.description.tableofcontentsPlease refer to README file for information about the data contained in each file.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. Quantitative Biology, Biochemistry and Biotechnologyen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1101/2020.02.03.932228en_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectSuper-resolutionen_UK
Subjectdc.subjectLive cell imagingen_UK
Subjectdc.subjectFluorescence microscopyen_UK
Subjectdc.subjectNanobiotechnologyen_UK
Subjectdc.subjectPeptide-binding moduleen_UK
Subjectdc.subjectCoiled coilen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleLIVE-PAINT Supporting Datasetsen_UK
Typedc.typedataseten_UK

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