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Depositordc.contributorWard, Paulineen
Funderdc.contributor.otherCunningham Trust
Funderdc.contributor.otherNational Natural Science Foundation of China
Funderdc.contributor.otherMinistry of Science and Technology Republic of China
Funderdc.contributor.otherHuman Frontiers Program
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Council
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Council
Funderdc.contributor.otherMRC - Medical Research Council
Data Creatordc.creatorMancini, Leonardo
Data Creatordc.creatorTerradot, Guillaume
Data Creatordc.creatorTian, Tian
Data Creatordc.creatorPu, YingYing
Data Creatordc.creatorLi, YingXing
Data Creatordc.creatorLo, Chien-Jung
Data Creatordc.creatorBai, Fan
Data Creatordc.creatorPilizota, Teuta
Date Accessioneddc.date.accessioned2020-05-19T11:07:45Z
Date Availabledc.date.available2020-05-19T11:07:45Z
Citationdc.identifier.citationMancini, Leonardo; Terradot, Guillaume; Tian, Tian; Pu, YingYing; Li, YingXing; Lo, Chien-Jung; Bai, Fan; Pilizota, Teuta. (2020). A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology, [dataset]. University of Edinburgh. School of Biological Sciences. https://doi.org/10.7488/ds/2825.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3635
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2825
Dataset Description (abstract)dc.description.abstractThe electrical membrane potential (Vm) is one of the components of the electrochemical potential of protons across the biological membrane (proton motive force), which powers many vital cellular processes. Because Vm also plays a role in signal transduction, measuring it is of great interest. Over the years, a variety of techniques have been developed for the purpose. In bacteria, given their small size, Nernstian membrane voltage probes are arguably the favorite strategy, and their cytoplasmic accumulation depends on Vm according to the Nernst equation. However, a careful calibration of Nernstian probes that takes into account the tradeoffs between the ease with which the signal from the dye is observed and the dyes’ interactions with cellular physiology is rarely performed. Here, we use a mathematical model to understand such tradeoffs and apply the results to assess the applicability of the Thioflavin T dye as a Vm sensor in Escherichia coli. We identify the conditions in which the dye turns from a Vm probe into an actuator and, based on the model and experimental results, propose a general workflow for the characterization of Nernstian dye candidates.
Dataset Description (TOC)dc.description.tableofcontents* "Figures_data.xlsx" contains values of the data points plotted. * Zip files contain raw data (images and Excel spreadsheets) relating to figures 3,4,5,SI7 and SI10 respectively.
Publisherdc.publisherUniversity of Edinburgh. School of Biological Sciences
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1016/j.bpj.2019.10.030
Relation (Is Referenced By)dc.relation.isreferencedbyMancini, L, Terradot, G, Tian, T, Pu, Y, Li, Y, Lo, C-J, Bai, F and Pilizota, T 2020, 'A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology', Biophysical Journal, vol. 118, no. 1, pp. 4-14. https://doi.org/10.1016/j.bpj.2019.10.030
Subjectdc.subjectNernstian probes
Subjectdc.subjectMembrane voltage
Subjectdc.subjectsingle-cell microscopy
Subjectdc.subjectMembrane voltage measurements
Titledc.titleA General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology
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