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Depositordc.contributorOi, Curran
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherNIH - National Institutes of Healthen_UK
Funderdc.contributor.otherUniversity of Edinburghen_UK
Funderdc.contributor.otherThe Euan MacDonald Centreen_UK
Funderdc.contributor.otherDr. Jim Love and UCB Pharma
Funderdc.contributor.otherUK Dementia Research Institute
Data Creatordc.creatorOi, Curran
Data Creatordc.creatorHorrocks, Mathew
Data Creatordc.creatorRegan, Lynne
Date Accessioneddc.date.accessioned2020-04-07T16:11:39Z
Date Availabledc.date.available2020-04-07T16:11:39Z
Citationdc.identifier.citationOi, Curran; Horrocks, Mathew; Regan, Lynne. (2020). LIVE-PAINT Supplementary Videos, [dataset]. University of Edinburgh. Quantitative Biology, Biochemistry and Biotechnology. https://doi.org/10.7488/ds/2801.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3610
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2801
Dataset Description (abstract)dc.description.abstractWe present LIVE-PAINT, a new approach to super-resolution fluorescent imaging inside live cells. In LIVE-PAINT only a short peptide sequence is fused to the protein being studied, unlike conventional super-resolution methods, which rely on directly fusing the biomolecule of interest to a large fluorescent protein, organic fluorophore, or oligonucleotide. LIVE-PAINT works by observing the blinking of localized fluorescence as this peptide is reversibly bound by a protein that is fused to a fluorescent protein. We have demonstrated the effectiveness of LIVE-PAINT by imaging a number of different proteins inside live S. cerevisiae. Not only is LIVE-PAINT widely applicable, easily implemented, and the modifications minimally perturbing, but we also anticipate it will extended data acquisition times compared to those previously possible with methods that involve direct fusion to a fluorescent protein. Please note, compressed versions of the mp4 files are available at https://doi.org/10.7488/ds/2839 .en_UK
Dataset Description (TOC)dc.description.tableofcontents# FILE LISTING # * "Supplementary Movie 1 - mKO blinking.avi" LIVE-PAINT blinking behavior shown in S. cerevisiae using the fluorescent protein mKO and the reversible interaction pair SYNZIP17-SYNZIP18. Scale bar is 1 micron. * "Supplementary Movie 2 - mOrange blinking.avi" LIVE-PAINT blinking behavior shown in S. cerevisiae using the fluorescent protein mOrange and the reversible interaction pair SYNZIP17-SYNZIP18. Scale bar is 1 micron. * "Supplementary Movie 3 - cofilin tracking.avi" Video tracking cofilin in S. cerevisiae, where cofilin is C-terminally tagged with SYNZIP18 and labeled with separately expressed SYNZIP17-mNeonGreen. Scale bar is 5 microns. * "Supplementary Movie 4 - cofilin tracking.avi" Tracking the diffusion of a single cofilin "spot" in S. cerevisiae, where cofilin is C-terminally tagged with SYNZIP18 and labeled with separately expressed SYNZIP17-mNeonGreen. Scale bar is 1 micron. MP4 versions of each file have also been included, for accessibility.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. Quantitative Biology, Biochemistry and Biotechnologyen_UK
Relation (Is Version Of)dc.relation.isversionofhttps://doi.org/10.7488/ds/2839
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1101/2020.02.03.932228en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyLIVE-PAINT: Super-Resolution Microscopy Inside Live Cells Using Reversible Peptide-Protein Interactions Curran Oi, Zoe Gidden, Louise Holyoake, Owen Kantelberg, Simon Mochrie, Mathew H. Horrocks, Lynne Regan bioRxiv 2020.02.03.932228; doi: https://doi.org/10.1101/2020.02.03.932228en_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectSuper-resolutionen_UK
Subjectdc.subjectLive cell imagingen_UK
Subjectdc.subjectFluorescence microscopyen_UK
Subjectdc.subjectNanobiotechnologyen_UK
Subjectdc.subjectPeptide-binding moduleen_UK
Subjectdc.subjectCoiled coilen_UK
Subjectdc.subjectPeptideen_UK
Subjectdc.subjectProteinen_UK
Subjectdc.subjectSaccharomyces cerevisiaeen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleLIVE-PAINT Supplementary Videosen_UK
Typedc.typedataseten_UK

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