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Depositordc.contributorJones, Anita
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Data Creatordc.creatorJones, Anita
Data Creatordc.creatorPaterson, Kyle
Data Creatordc.creatorArlt, Jochen
Date Accessioneddc.date.accessioned2019-12-03T14:45:43Z
Date Availabledc.date.available2019-12-03T14:45:43Z
Citationdc.identifier.citationJones, Anita; Paterson, Kyle; Arlt, Jochen. (2019). Dynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solution, [dataset]. University of Edinburgh. School of Chemistry. https://doi.org/10.7488/ds/2717.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3516
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2717
Dataset Description (abstract)dc.description.abstract2-aminopurine (2AP) is a responsive fluorescent base analogue that is used widely as a probe of the local molecular environment in DNA. The ability of 2AP to report changes in local conformation and base-stacking interactions arises from the efficient quenching of its fluorescence by the natural DNA bases. However, the mechanism of this inter-base quenching remains imperfectly understood. Two previous studies of the collisional quenching of 2AP by the natural bases, in different buffer solutions, showed that dynamic quenching efficiency depends on the identity of the natural base, but disagreed on the relative quenching efficiencies of the bases. We report a comprehensive investigation of inter-base quenching of 2AP by the natural nucleoside monophosphates (NMPs), replicating the buffer conditions used in the previous studies. Using time-resolved fluorescence measurements to distinguish between dynamic and static quenching, we find that the dynamic quenching rate constants of the different bases show a consistent trend across both buffers, and this is in line with a charge-transfer mechanism. Time-resolved measurements also provide insight into static quenching, revealing formation of 2AP-NMP ground-state complexes in which 2AP displays a very short fluorescence lifetime, comparable to that seen in oligonucleotides. In these complexes, the dependence of the rate of quenching on the partner base also supports a charge-transfer mechanism.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Chemistryen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyKyle A. Paterson, Jochen Arlt and Anita C. Jones. Dynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solution. Methods and Applications in Fluorescence.(manuscript in submission)en_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Titledc.titleDynamic and static quenching of 2-aminopurine fluorescence by the natural DNA nucleotides in solutionen_UK
Typedc.typedataseten_UK

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