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Depositordc.contributorBurdon, Tomen
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Council
Data Creatordc.creatorBurdon, Tom
Data Creatordc.creatorMeek, Stephen
Data Creatordc.creatorJoshi, Anagha
Data Creatordc.creatorChandra, Tamir
Date Accessioneddc.date.accessioned2019-11-01T09:31:43Z
Date Availabledc.date.available2019-11-01T09:31:43Z
Citationdc.identifier.citationBurdon, Tom; Meek, Stephen; Joshi, Anagha; Chandra, Tamir. (2019). Single cell transcriptional profiling of rat embryonic stem cells defined by Rex1-EGFP reporter expression, [dataset]. University of Edinburgh. Roslin Institute. https://doi.org/10.7488/ds/2639.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3436
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2639
Dataset Description (abstract)dc.description.abstractRat embryonic stem cells exhibit basal levels of spontaneous differentiation in 2i+LIF (2iL) culture. Expression of a stem cell specific Rex1-EGFP knock-in reporter transgene in rat ESCs was used to isolate functionally distinct subsets of cells in rat ESC cultures, and determine their transcriptional profiles by single cell RNA sequencing.
Dataset Description (TOC)dc.description.tableofcontentsFiles included in this dataset: (1) Methodology (document including a diagram) (2) A tar file "plates.tar" containing 385 folders containing Single cell RNA sequencing fastq.gz files (to open, untar the .tar file, then gunzip the .gz files).
Publisherdc.publisherUniversity of Edinburgh. Roslin Institute
Relation (Is Referenced By)dc.relation.isreferencedbyStephen Meek, Jun Wei, Taeho Oh, Tom Watson, Jaime Olavarrieta, Linda Sutherland, Daniel F. Carlson , Angela Salzano, Tamir Chandra, Anagha Joshi and Tom Burdon "A stem cell reporter for investigating pluripotency and self-renewal in the rat" Stem Cell Reports, in submission
Subjectdc.subjectrat
Subjectdc.subjectRex1
Subjectdc.subjectpluripotency
Subjectdc.subjectsingle cell RNA sequencing
Subjectdc.subjectEmbryonic stem cells
Subject Classificationdc.subject.classificationBiological Sciences
Titledc.titleSingle cell transcriptional profiling of rat embryonic stem cells defined by Rex1-EGFP reporter expression
Typedc.typedataset

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