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Depositordc.contributorMcCulloch, Laura
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Funderdc.contributor.otherUniversity of Edinburghen_UK
Data Creatordc.creatorMcCulloch, Laura
Date Accessioneddc.date.accessioned2019-09-30T10:00:13Z
Date Availabledc.date.available2019-09-30T10:00:13Z
Citationdc.identifier.citationMcCulloch, Laura. (2019). Interleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence, [dataset]. UK Dementia Research Institute at the University of Edinburgh. https://doi.org/10.7488/ds/2626.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3422
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2626
Dataset Description (abstract)dc.description.abstractBackground: Blockade of the cytokine interleukin-1 (IL-1) with IL-1 receptor antagonist (IL-1Ra) is a candidate treatment for stroke entering phase II/III trials, which acts by inhibiting harmful inflammatory responses. Infection is a common complication after stroke that significantly worsens outcome and is related to stroke-induced deficits in systemic immune function thought to be mediated by the sympathetic nervous system. Therefore, immunomodulatory treatments for stroke, such as IL-1Ra, carry a risk of aggravating stroke-associated infection. Our primary objective was to determine if factors associated with antibody-mediated antibacterial defences were further compromised in patients treated with IL-1Ra after stroke. Methods: We assessed plasma concentrations of immunoglobulin isotypes and complement components in stroke patients treated with IL-1Ra or placebo and untreated non-stroke controls using multiplex protein assays. Activation of the sympathetic nervous system (SNS) was determined by measuring noradrenaline, a major SNS mediator. Results: There were significantly lower plasma concentrations of IgM, IgA, IgG1 and IgG4 in stroke-patients compared to non-stroke controls, however there were no differences between stroke patients treated with placebo or IL-1Ra. Concentrations of complement components associated with the classical pathway were increased and those associated with the alternative pathways decreased in stroke patients, neither being affected by treatment with IL-1Ra. Noradrenaline concentrations were increased after stroke in both placebo and IL-1Ra-treated stroke patients compared to non-stroke controls. Conclusion: These data show treatment with IL-1Ra after stroke does not alter circulating immunoglobulin and complement concentrations and is therefore unlikely to further aggravate stroke-associated infection susceptibility through reduced altered availability of these key anti-microbial mediators. Supplementary Figures from the manuscript are also available here.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUK Dementia Research Institute at the University of Edinburghen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.12688/f1000research.19308.1
Relation (Is Referenced By)dc.relation.isreferencedbyMcCulloch, L, McColl, B, Allan, SM, Emsley, HCA & Smith, CJ 2019, 'Interleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence', F1000Research. https://doi.org/10.12688/f1000research.19308.1
Relation (Is Referenced By)dc.relation.isreferencedbyInterleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence. Laura McCulloch, Stuart M. Allan, Craig J. Smith, Barry W. McColl. bioRxiv 587881; doi: https://doi.org/10.1101/587881
Supersedesdc.relation.replaceshttps://doi.org/10.7488/ds/2570en_UK
Supersedesdc.relation.replaceshttps://doi.org/10.7488/ds/2545
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectstrokeen_UK
Subjectdc.subjectIL-1Raen_UK
Subjectdc.subjectimmunoglobulinsen_UK
Subjectdc.subjectcomplementen_UK
Subjectdc.subjectinfectionen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleInterleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defenceen_UK
Typedc.typedataseten_UK

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