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Depositordc.contributorMcCulloch, Laura
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Funderdc.contributor.otherUniversity of Edinburghen_UK
Spatial Coveragedc.coverage.spatialUKen
Spatial Coveragedc.coverage.spatialUNITED KINGDOMen
Data Creatordc.creatorMcCulloch, Laura
Date Accessioneddc.date.accessioned2019-05-10T12:30:36Z
Date Availabledc.date.available2019-05-10T12:30:36Z
Citationdc.identifier.citationMcCulloch, Laura. (2019). Supplementary Figures: Interleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence, [dataset]. University of Edinburgh. UK Dementia Research Institute. https://doi.org/10.7488/ds/2545.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3323
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2545
Dataset Description (abstract)dc.description.abstractSupplementary Figures from the manuscript "Interleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence". ## ABSTRACT ## ### Background ### Blockade of the cytokine interleukin-1 (IL-1) with IL-1 receptor antagonist (IL-1Ra) is a candidate treatment for stroke entering phase II/III trials, which acts by inhibiting harmful inflammatory responses. Infection is a common complication after stroke that significantly worsens outcome and is related to stroke-induced deficits in systemic immune function thought to be mediated by the sympathetic nervous system. Therefore, immunomodulatory treatments for stroke, such as IL-1Ra, carry a risk of aggravating stroke-associated infection. Our primary objective was to determine if factors associated with antibody-mediated antibacterial defences were further compromised in patients treated with IL-1Ra after stroke. ### Methods ### We assessed plasma concentrations of immunoglobulin isotypes and complement components in stroke patients treated with IL-1Ra or placebo and untreated non-stroke controls using multiplex protein assays. Activation of the SNS was determined by measuring noradrenaline, a major SNS mediator. ### Results ### There were significantly lower plasma concentrations of IgM, IgA, IgG1 and IgG4 in stroke-patients compared to non-stroke controls, however there were no differences between stroke patients treated with placebo or IL-1Ra. Concentrations of complement components associated with the classical pathway were increased and those associated with the alternative pathways decreased in stroke patients, neither being affected by treatment with IL-1Ra. Noradrenaline concentrations were increased after stroke in both placebo and IL-1Ra-treated stroke patients compared to non-stroke controls. ### Conclusion ### These data show treatment with IL-1Ra after stroke does not alter circulating immunoglobulin and complement concentrations and is therefore unlikely to further aggravate stroke-associated infection susceptibility through reduced availability of these key anti-microbial mediators.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUK Dementia Research Institute at the University of Edinburghen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1101/587881en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyInterleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defence. Laura McCulloch, Stuart M. Allan, Craig J. Smith, Barry W. McColl. bioRxiv 587881; doi: https://doi.org/10.1101/587881
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subject Classificationdc.subject.classificationMedicine and Dentistry::Clinical Medicineen_UK
Titledc.titleSupplementary Figures: Interleukin-1 receptor antagonist treatment in acute ischaemic stroke does not alter systemic markers of anti-microbial defenceen_UK
Typedc.typedataseten_UK

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