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Depositordc.contributorJia, Jiabin
Funderdc.contributor.otherUniversity of Edinburghen_UK
Data Creatordc.creatorWu, Hancong
Data Creatordc.creatorYang, Yunjie
Data Creatordc.creatorBagnaninchi, Pierre
Data Creatordc.creatorJia, Jiabin
Date Accessioneddc.date.accessioned2019-04-16T13:02:54Z
Date Availabledc.date.available2019-04-16T13:02:54Z
Citationdc.identifier.citationWu, Hancong; Yang, Yunjie; Bagnaninchi, Pierre; Jia, Jiabin. (2019). Datasets of journal paper "Electrical impedance tomography for real-time and label-free cellular viability assays of 3D tumour spheroids", [dataset]. University of Edinburgh. School of Engineering. Institute for Digital Communications. https://doi.org/10.7488/ds/2534.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3310
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2534
Dataset Description (abstract)dc.description.abstractThis dataset comprises raw voltage measurement data to reconstruct images in Fig 6 in paper "Electrical impedance tomography for real-time and label-free cellular viability assays of 3D tumour spheroids". ABSTRACT: There is currently a need to culture cells in 3D to better mimic the behaviour of cells growing in the natural environment. In parallel, this calls for novel technologies to assess cell growth in 3D cell culture. In this study, we demonstrated both in silico and in vitro that cell viability inside large cell spheroids could be monitored in real time and label-free with electrical impedance tomography (EIT). Simulations using a single shell model and the effective media approximation (EMA) method were performed to prove the performance of EIT on spheroid imaging and viability monitoring. Then in vitro experiments were conducted to measure in real time a loss of cell viability in MCF-7 breast cancer spheroids when exposed to Triton X-100 and validate with conventional biochemical assays. It is shown that EIT has a spatial resolution of 1.14% and it could monitor the cell mortality over 20% of a spheroid under laboratory noise level. The reconstructed conductivity images for cell mortality induced by the chemical are clear and match the result in the cellular metabolic viability assay. Furthermore, the image reconstruction speed in the experiment was less than 0.3 seconds. Taken together, the results show the potential of EIT for non-destructive real-time and label-free cellular assays in the miniature sensor, providing physiological information in the applications of 3D drug screening and tissue engineering.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Engineering. Institute for Digital Communicationsen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.3390/ma11060930en_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectelectrical impedance tomographyen_UK
Subjectdc.subjectcell viabilityen_UK
Subjectdc.subjectreal-timeen_UK
Subjectdc.subject3D tumour spheroidsen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleDatasets of journal paper "Electrical impedance tomography for real-time and label-free cellular viability assays of 3D tumour spheroids"en_UK
Typedc.typedataseten_UK

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