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Depositordc.contributorHulme, Alison
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Funderdc.contributor.otherWellcome Trusten_UK
Data Creatordc.creatorSim, Alisia M
Data Creatordc.creatorRashdan, Nabil A
Data Creatordc.creatorCui, Lin
Data Creatordc.creatorMoss, Alistair J
Data Creatordc.creatorNudelman, Fabio
Data Creatordc.creatorDweck, Marc R
Data Creatordc.creatorMacRae, Vicky E
Data Creatordc.creatorHulme, Alison N
Date Accessioneddc.date.accessioned2018-11-09T15:18:12Z
Date Availabledc.date.available2018-11-09T15:18:12Z
Citationdc.identifier.citationSim, Alisia M; Rashdan, Nabil A; Cui, Lin; Moss, Alistair J; Nudelman, Fabio; Dweck, Marc R; MacRae, Vicky E; Hulme, Alison N. (2018). A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models, [dataset]. University of Edinburgh. School of Chemistry. http://dx.doi.org/10.7488/ds/2467.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3216
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/2467
Dataset Description (abstract)dc.description.abstractA rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Chemistryen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1038/s41598-018-35454-9en_UK
Relation (Is Referenced By)dc.relation.isreferencedbySim, AM, Rashdan, NA, Cui, L, Moss, AJ, Nudelman, F, Dweck, MR, Macrae, VE & Hulme, AN 2018, 'A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models' Scientific Reports, vol. 8, no. 1, pp. 17360. DOI: 10.1038/s41598-018-35454-9
Subjectdc.subjectfluoresence imagingen_UK
Subjectdc.subjecthydroxyapatiteen_UK
Subjectdc.subjectcalcificationen_UK
Subjectdc.subjectatherosclerosisen_UK
Subject Classificationdc.subject.classificationPhysical Sciences::Medicinal Chemistryen_UK
Titledc.titleA novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue modelsen_UK
Typedc.typedataseten_UK

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