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Depositordc.contributorJones, Anita
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Funderdc.contributor.otherUniversity of Edinburghen_UK
Data Creatordc.creatorJones, Anita C
Data Creatordc.creatorMagennis, Steven W
Date Accessioneddc.date.accessioned2018-10-24T11:37:52Z
Date Availabledc.date.available2018-11-01T05:15:07Z
Citationdc.identifier.citationJones, Anita C; Magennis, Steven W. (2018). Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity, [dataset]. University of Edinburgh. School of Chemistry. https://doi.org/10.7488/ds/2457.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3202
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2457
Dataset Description (abstract)dc.description.abstractThese data are associated with the paper "Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity", Rachel S. Fisher, David Nobis, Anders F. Füchtbauer, Mattias Bood, Morten Grøtli, L. Marcus Wilhelmsson, Anita C. Jones and Steven W. Magennis. Fluorescent nucleobase analogues (FBAs) have many desirable features in comparison to extrinsic fluorescent labels, but they are yet to find application in ultrasensitive detection. Many of the disadvantages of FBAs arise from their short excitation wavelengths (often in the ultraviolet), making two-photon excitation a potentially attractive approach. Pentacyclic adenine (pA) is a recently developed FBA that has an exceptionally high two-photon brightness. We have studied the two-photon-excited fluorescence properties of pA and how they are affected by incorporation in DNA. We find that pA is more photostable under two-photon excitation than via resonant absorption. When incorporated in an oligonucleotide, pA has a high two-photon cross section and emission quantum yield, varying with sequence context, resulting in the highest reported brightness for such a probe. The use of a two-photon microscope with ultrafast excitation and pulse shaping has allowed the detection of pA-containing oligonucleotides in solution with a limit of detection of ~ 5 molecules, demonstrating that practical single-molecule detection of FBAs is now within reach.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Chemistryen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1039/C8CP05496G
Relation (Is Referenced By)dc.relation.isreferencedbyFisher, RS, Nobis, D, Füchtbauer, AF, Bood, M, Grøtli, M, Wilhelmsson, LM, Jones, AC & Magennis, SW 2018, 'Pulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivity' Physical chemistry chemical physics. DOI: 10.1039/C8CP05496G
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectTwo-photon spectroscopyen_UK
Subjectdc.subjectDNAen_UK
Subjectdc.subjectbase analogueen_UK
Subjectdc.subjectsingle moleculeen_UK
Subjectdc.subjectfluorescenceen_UK
Subjectdc.subjectFCSen_UK
Subject Classificationdc.subject.classificationPhysical Sciences::Chemistryen_UK
Titledc.titlePulse-shaped two-photon excitation of a fluorescent base analogue approaches single-molecule sensitivityen_UK
Typedc.typedataseten_UK

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