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Depositordc.contributorRegan, Tim
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Spatial Coveragedc.coverage.spatialScotlanden_UK
Spatial Coveragedc.coverage.spatialEnglanden_UK
Spatial Coveragedc.coverage.spatialUKen
Spatial Coveragedc.coverage.spatialUNITED KINGDOMen
Time Perioddc.coverage.temporalstart=2016-10-01; end=2017-06-01; scheme=W3C-DTFen
Data Creatordc.creatorRegan, Tim
Data Creatordc.creatorFreeman, Tom
Data Creatordc.creatorBarnett, Mark
Date Accessioneddc.date.accessioned2018-10-11T11:06:28Z
Date Availabledc.date.available2018-10-15T04:15:09Z
Citationdc.identifier.citationRegan, Tim; Freeman, Tom; Barnett, Mark. (2018). Characterisation of the UK honey bee metagenome, 2016-2017 [dataset]. The Roslin Institute. University of Edinburgh. https://doi.org/10.7488/ds/2453.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/3197
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2453
Dataset Description (abstract)dc.description.abstractThe European honey bee (Apis mellifera) plays a major role in pollination and food production, but is under threat from emerging pathogens and agro-environmental insults. As with other organisms, honey bee health is a complex product of environment, host genetics and associated microbes (commensal, opportunistic and pathogenic). Improved understanding of bee genetics and their molecular ecology can help manage modern challenges to bee health and production. Sampling bee and cobiont genomes, we characterised the metagenome of 19 honey bee colonies across Britain. Low heterozygosity was observed in bees from many Scottish colonies, sharing high similarity to the native dark bee, A. mellifera mellifera. Apiaries exhibited high diversity in the composition and relative abundance of individual microbiome taxa. Most non-bee sequences derived from known honey bee commensal bacteria or known pathogens, e.g. Lotmaria passim (Trypanosomatidae), and Nosema spp. (Microsporidia). However, DNA was also detected from numerous additional bacterial, plant (food source), protozoan and metazoan organisms. To classify sequences from cobionts lacking genomic information, we developed a novel network analysis approach clustering orphan contigs, allowing the identification of a pathogenic gregarine. Our analyses demonstrate the power of high-throughput, directed metagenomics in agroecosystems identifying potential threats to honey bees present in their microbiota.en_UK
Dataset Description (TOC)dc.description.tableofcontentsContains raw Illumina reads, assembled contigs, annotated clusters of contigs. View ReadMe.txt for description of files.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. The Roslin Instituteen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1038/s41467-018-07426-0en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyRegan, T, Barnett, MW, Laetsch, DR, Bush, SJ, Wragg, D, Budge, GE, Highet, F, Dainat, B, De Miranda, JR, Watson, M, Blaxter, M & Freeman, TC 2018, 'Characterisation of the British honey bee metagenome' Nature Communications, vol. 9, no. 1. DOI: 10.1038/s41467-018-07426-0en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://www.biorxiv.org/content/early/2018/05/18/293647
Relation (Is Referenced By)dc.relation.isreferencedbyCharacterisation of the UK honey bee (Apis mellifera) metagenome. Tim Regan, Mark W Barnett, Dominik R Laetsch, Stephen J Bush, David Wragg, Giles E Budge, Fiona Highet, Benjamin Dainat, Joachim R de Miranda, Mark Blaxter, Tom C Freeman bioRxiv 293647; doi: https://doi.org/10.1101/293647
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectMetagenomicsen_UK
Subjectdc.subjectgenomicsen_UK
Subjectdc.subjectmicrobiomeen_UK
Subjectdc.subjectHoney beeen_UK
Subjectdc.subjectApis melliferaen_UK
Subjectdc.subjectNetwork analysisen_UK
Subjectdc.subjectNetwork graphen_UK
Subjectdc.subjectAssembliesen_UK
Subject Classificationdc.subject.classificationBiological Sciences::Genetics not elsewhere classifieden_UK
Titledc.titleCharacterisation of the UK honey bee metagenomeen_UK
Typedc.typedataseten_UK

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