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Depositordc.contributorTaylor, Martin
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Data Creatordc.creatorYoung, Robert
Data Creatordc.creatorTaylor, Martin
Date Accessioneddc.date.accessioned2017-12-12T09:46:35Z
Date Availabledc.date.available2017-12-21
Citationdc.identifier.citationYoung, Robert; Taylor, Martin. (2017). Bidirectional transcription initiation marks accessible chromatin and is not specific to enhancers, [text]. University of Edinburgh. MRC IGMM. MRC Human Genetics Unit. https://doi.org/10.7488/ds/2266en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2972
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2266
Dataset Description (abstract)dc.description.abstractEnhancers are modular regulatory elements that are central to the spatial and temporal regulation of gene expression. Bidirectional transcription initiating at enhancers has been proposed to mark active enhancers and as such has been utilized to experimentally identify active enhancers de novo. Here, we show that bidirectional transcription initiation is a pervasive feature of accessible chromatin, including at enhancers, promoters, and other DNase hypersensitive regions not marked with canonical histone modification profiles. Transcription is less predictive for enhancer activity than epigenetic modifications such as H3K4me1 or the accessibility of DNA when measured in both enhancer assays and at endogenous loci. The stability of enhancer initiated transcripts does not influence measures of enhancer activity and we cannot detect evidence of purifying selection on the resulting enhancer RNAs within the human population. Our results indicate that bidirectional transcription initiation from accessible chromatin is not sufficient for, nor specific to, enhancer activity. Transcription initiating at enhancers may be a frequent by-product of promiscuous RNA polymerase initiation at accessible chromatin and is unlikely to generally play a functional role in enhancer activity.en_UK
Dataset Description (TOC)dc.description.tableofcontentsSee README.txten_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. MRC IGMM. MRC Human Genetics Uniten_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1101/048629en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyYoung, Robert S; Kumar, Yatendra; Bickmore, Wendy A; Taylor, Martin S "Bidirectional transcription marks accessible chromatin and is not specific to enhancers" BioRXiv doi: https://doi.org/10.1101/048629
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectenhanceren_UK
Subjectdc.subjectgenomeen_UK
Subjectdc.subjecttranscriptionen_UK
Subjectdc.subjecteRNAen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleBidirectional transcription initiation marks accessible chromatin and is not specific to enhancersen_UK
Typedc.typetexten_UK

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