Granneman, Sander. (2017). Yeast 18S rRNA DMS RNA structure probing ChemModSeq data, [dataset]. University of Edinburgh. School of Biological Sciences. https://doi.org/10.7488/ds/2224.
These fasta files were generated from fastq files from the Hector et al NAR 2014 ChemModSeq paper ( https://academic.oup.com/nar/article-lookup/doi/10.1093/nar/gku815 ).
Included are biological triplicates of 18S DMS RNA structure probing data.
The data were generated using the ChemModSeq pipeline (https://bitbucket.org/sgrann/chemmodseqpipeline).
The reads from the raw fastq files were trimmed using FlexBar to remove adapters and low quality nucleotides. The sequence information present in the in-read random barcodes was used to remove PCR duplicates. Thus the data in the fasta files represent unique cDNAs. The first nucleotide in the forward (_1) read data is the reverse transcriptase drop-off point.
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