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Depositordc.contributorKleinjan, Dirk-Jan
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Data Creatordc.creatorRosser, Susan
Data Creatordc.creatorKleinjan, Dirk-Jan
Date Accessioneddc.date.accessioned2017-08-24T11:22:50Z
Date Availabledc.date.available2017-12-31T05:15:11Z
Citationdc.identifier.citationRosser, Susan; Kleinjan, Dirk-Jan. (2017). Data in support of 'Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors', [dataset]. University of Edinburgh, UK Centre for Mammalian Synthetic Biology, School of Biological Sciences. https://doi.org/10.7488/ds/2121.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2798
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/2121
Dataset Description (abstract)dc.description.abstractThis dataset contains a file with images of the full Western blots used in the manuscript "Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors", plus a file with DNA sequences of constructs used in the study. The nuclease-deactivated variant of CRISPR/Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9/Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh, UK Centre for Mammalian Synthetic Biology, School of Biological Sciencesen_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectCrispr/dCas9en_UK
Subjectdc.subjectDrug-inducible Degronen_UK
Subjectdc.subjectGene Regulationen_UK
Subjectdc.subjectWestern blotsen_UK
Subjectdc.subjectqPCRen_UK
Subjectdc.subjectLuciferase assaysen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleData in support of 'Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors'en_UK
Typedc.typedataseten_UK

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