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Depositordc.contributorRibchester, Richard R.
Depositordc.contributorWard, Paulineen
Funderdc.contributor.otherMRC - Medical Research Council
Funderdc.contributor.otherNorth British Hotels Trust
Funderdc.contributor.otherMotor Neurone Disease Association
Data Creatordc.creatorHirst, Theodore C.
Data Creatordc.creatorRibchester, Richard R.
Date Accessioneddc.date.accessioned2017-06-30T11:25:32Z
Date Availabledc.date.available2017-06-30T11:25:32Z
Citationdc.identifier.citationHirst, Theodore C.; Ribchester, Richard R. (2017). Segmentation of the 4DL Connectome 1: Data analysis, [dataset]. University of Edinburgh. Centre for Integrative Physiology. http://dx.doi.org/10.7488/ds/2080.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2749
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/2080
Dataset Description (abstract)dc.description.abstractConnectomic analysis of the nervous system aims to discover and establish principles that underpin normal and abnormal neural connectivity and function. In Hirst et al (2013) we performed image analysis of motor unit connectivity in the fourth deep lumbrical muscle (4DL) of mice, using transgenic expression of fluorescent protein in motor neurones as a morphological reporter. We developed a method that accelerated segmentation of confocal image projections of 4DL motor units, by applying high resolution (63×, 1.4 NA objective) imaging or deconvolution only where either proved necessary, in order to resolve axon crossings that produced ambiguities in the correct assignment of axon terminals to identified motor units imaged at lower optical resolution (40×, 1.3 NA). This dataset contains files with the major analyses done for the paper, in particular global_analysis.pzf – generated in Graphpad Prism. The remainder of the data relating to this study can be found grouped together in the "Connectomic analysis of the mouse fourth deep lumbrical muscle connectome" DataShare Collection at http://datashare.is.ed.ac.uk/handle/10283/2657.
Publisherdc.publisherUniversity of Edinburgh. Centre for Integrative Physiology
Relation (Is Referenced By)dc.relation.isreferencedbyhttp://onlinelibrary.wiley.com/doi/10.1113/jphysiol.2013.258087/abstract
Relation (Is Referenced By)dc.relation.isreferencedbyHirst, TC; Ribchester, RR (2013)"Segmentation of the mouse fourth deep lumbrical muscle connectome reveals concentric organisation of motor units" J. Physiol, DOI: 10.1113/jphysiol.2013.258087
Subjectdc.subjectNMJ
Subjectdc.subjectneuromuscular junction
Subjectdc.subjectsynapse
Subjectdc.subjectconnectomic analysis
Subjectdc.subjectconnectomics
Subjectdc.subjectmouse
Subjectdc.subjectmuscle
Subjectdc.subjectmotor unit
Subjectdc.subjectconfocal microscopy
Subjectdc.subjectend-plate
Subjectdc.subjectmotor nerve
Subjectdc.subjectaxon
Subjectdc.subjectfluorescent protein
Subjectdc.subjectbungarotoxin
Subjectdc.subjectmotor unit size
Subject Classificationdc.subject.classificationSubjects allied to Medicine::Neuroscience
Titledc.titleSegmentation of the 4DL Connectome 1: Data analysis
Typedc.typedataset

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