Mills, Bethany; Megia-Fernandez, Alicia. (2017). Dataset for paper 'Highly Selective and Rapidly Activatable Fluorogenic Thrombin Sensors and Application in Human Lung Tissue', [dataset]. University of Edinburgh. School of Chemistry. https://doi.org/10.7488/ds/2006.
Dataset related to publication DOI: 10.1039/c7ob00663b.
A library of FRET-based peptides were prepared and studied as Thrombin substrates. This identified probes that showed selective activation by Thrombin, low background signal, stability to Factor Xa, matrix metalloproteases, and primary human inflammatory cell lysates and supernatant. These were selected for further optimization, creating a second generation of fluorogenic probes with improved solubility and Plasmin resistance. The optimized probe detected Thrombin activity ex vivo in fibrotic human lung tissue.
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