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Depositordc.contributorBromley, Keith Matthew
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Spatial Coveragedc.coverage.spatialEdinburghen_UK
Spatial Coveragedc.coverage.spatialUKen
Spatial Coveragedc.coverage.spatialUNITED KINGDOMen
Time Perioddc.coverage.temporalstart=2015-08-03; end=2016-09-09; scheme=W3C-DTFen
Data Creatordc.creatorBromley, Keith Matthew
Data Creatordc.creatorMacPhee, Cait
Date Accessioneddc.date.accessioned2016-12-19T10:55:59Z
Date Availabledc.date.available2016-12-19T10:55:59Z
Citationdc.identifier.citationBromley, Keith Matthew; MacPhee, Cait. (2016). BslA-stabilised emulsion droplets with designed microstructure, 2015-2016 [image]. University of Edinburgh. http://dx.doi.org/10.7488/ds/1590.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2229
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/1590
Dataset Description (abstract)dc.description.abstractOriginal microscope images of BslA-stabilised emulsion droplets and two time-lapse confocal microscope sequences (available to view using ImageJ imaging software) of BslA-stabilised partially coalesced droplets melting in the absence and presence of free BslA in the medium.en_UK
Dataset Description (TOC)dc.description.tableofcontentsFigure 1 files: Images of emulsion droplets arrested during emulsification. Figure1a_EMx63_vrtx_AxA-BslA_0-2mgmL_90uL_HD_10uL_VF0-1_vrtx15s_in_2mLvial_07_10x_SB.tif: Elongated droplets trapped during vortex mixing. Figure1b_EMx67_AxA_0-2mgml_HDNR_VF0-5_no_air_rollerbank_overnight_07_20x.tif: Rod-shaped droplets formed by rolling a vial of BslA and hexadecane on a rollerbank for 24 hours. Figure1c_160331_EMx67A_2016_03_31__12_05_35-ch1_FireLUT_SB.tif: Rod-shaped rollerbank droplets stained with Nile Red and viewed using confocal laser-scanning microscopy. Figure1d_EMx64_HD_flocs_AxA_0-2mgml_10_20x.tif: Rod-shaped oil droplets created by “floccing” BslA solution in a pipettor tip in the presence of hexadecane. Figure 2 files: Images of emulsion droplets arrested during emulsification. Figure2a_160921_EMx72_sonicated_AxA-BslA_0-2mgml_hexadecane_VF0-2_13_20x.tif: Hexadecane snowman droplets formed via emulsification using a probe sonicator. Figure2b_160212_EMx59D_AxA_0-2mgml_HD_VF0-2_RS15s_02_20x.tif: Hexadecane snowman droplets formed via emulsification using a high-shear mixer. Figure2c_150803_EMx20a_AxA-BslA_0-2mgml_GTO_VF0-5_RS1s_03_5x.tif: Amorphous blobs of hexadecane formed after ~1s of emulsification in BslA using a high shear mixer. Figure 3 files: Time-lapse sequences of melting partially coalesced BslA-stabilised emulsion droplets: Figure3a-b_160219_EMx60B_2016_02_19__13_24_05_melting_Mickey_40x_T-t-series.lsm AND Figure3c-d_160224_EMx60B_0-9mgml_AxA_in_medium_14C-20C_2016_02_24__16_49_53_t-T-series_0-5Cmin.lsm: Melting transitions of BslA stabilised partially coalesced frozen hexadecane droplets with (Figure3a-b) no excess BslA and (Figure3c-d) 0.9 mg/mL BslA in the continuous phase. Relaxation at the neck occurred more significantly in the absence of free BslA. The ramp rate was 0.5 °C/min. All scale bars = 10 μm. Figure 4 files: Microscope images of extruded BslA-stabilised cylindrical fat droplet before and after melting. Figure4a_EMx69_extruded_from_27G_needle_AxA_0-2mgml_cocoNR_13_5x_solid_SB.tif: Coconut fat rod-shaped emulsion drop formed by extruding the fat from a 27G needle into BslA solution at 5 °C. Figure4b_EMx69_extruded_from_27G_needle_AxA_0-2mgml_cocoNR_26_5x_melted_SB.tif: The same droplet after warming to 30 °C. Even after the fat had melted to oil, the overall morphology of the droplet was maintained by the elastic BslA film at the interface.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburghen_UK
Subjectdc.subjectBslAen_UK
Subjectdc.subjectEmulsionsen_UK
Subjectdc.subjectMicrostructureen_UK
Subjectdc.subjectInterfacial stabilisationen_UK
Subjectdc.subjectArrested coalescenceen_UK
Subject Classificationdc.subject.classificationPhysical Sciencesen_UK
Titledc.titleBslA-stabilised emulsion droplets with designed microstructureen_UK
Typedc.typeimageen_UK

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