Using the pimeloyl-CoA synthetase adenylation fold to synthesise fatty acid thioesters
Data CreatorWang, Menglu
PublisherUniversity of Edinburgh, School of Chemistry
Relation (Is Referenced By)https://doi.org/10.1038/nchembio.2361
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CitationWang, Menglu; Campopiano, Dominic; Pipier, Andrew; Kelly, Van; Harrison, Peter. (2016). Using the pimeloyl-CoA synthetase adenylation fold to synthesise fatty acid thioesters, [dataset]. University of Edinburgh, School of Chemistry. https://doi.org/10.7488/ds/1560.
DescriptionBiotin is an essential vitamin in plants and mammals functioning as the carbon dioxide carrier within central lipid metabolism. Bacterial pimeloyl-CoA synthetase (PCAS) acts as a highly specific substrate selection gate ensuring the integrity of the carbon chain in biotin synthesis. PCAS catalyses the condensation of pimelic acid (C7 dicarboxylic acid) with CoASH in an ATP dependent manner to form pimeloyl-CoA, the first dedicated biotin building block. Multiple structures of Bacillus subtilis PCAS together capture all three substrates as well as the intermediate pimeloyl-adenylate and product pyrophosphate (PPi). The enzyme uses an internal ruler to select the correct dicarboxylic acid substrate. Site directed mutagenesis has rationalised both the catalytic mechanism and the surprising stability of the adenylate intermediate. Building on this understanding, PCAS has been engineered to synthesise high value heptanoyl (C7) and octanoyl (C8) mono carboxylic acid-CoA and C8 dicarboxylic- CoA products highlighting the synthetic potential of PCAS. Data relating to the publication Wang et al.
Raw data relating to native mass spectrometry experiments of BioW with and without the addition of pimelic acid and magnesium ATP. Data require MassLynx V4 for analysis. (521.4Mb)
LC-MS chromatograms of incubations of BioW Y221F with fatty acid analogues. Data are the raw unprocessed file and require MassLynx for analysis. (1.498Gb)
Exported HPLC chromatograms of BioW WT and BioW Y221F incubations with hepatnoic acid/ATP/coenzyme-A (Fig. 5), ATP/ADP/AMP standards. (.txt format) (172.7Kb)
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