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Depositordc.contributorJafali, James
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherMRC - Medical Research Councilen_UK
Funderdc.contributor.otherWellcome Trusten_UK
Funderdc.contributor.otherBayGeneen_UK
Funderdc.contributor.otherScottish Enterprise
Funderdc.contributor.otherEuropean Union
Time Perioddc.coverage.temporalstart=2012-01; end=2013-08; scheme=W3C-DTFen
Data Creatordc.creatorGriffiths, Samantha
Data Creatordc.creatorKoegl, Manfred
Data Creatordc.creatorBoutell, Chris
Data Creatordc.creatorZenner, Helen
Data Creatordc.creatorCrump, Colin
Data Creatordc.creatorPica, Francesca
Data Creatordc.creatorGonzalez, Orland
Data Creatordc.creatorFriedel, Caroline
Data Creatordc.creatorBarry, Gerald
Data Creatordc.creatorMartin, Kim
Data Creatordc.creatorCraigon, Marie
Data Creatordc.creatorChen, Rui
Data Creatordc.creatorKaza, Lakshmi
Data Creatordc.creatorFossum, Even
Data Creatordc.creatorFazakerley, John
Data Creatordc.creatorEfstathiou, Stacey
Data Creatordc.creatorVolpi, Antonio
Data Creatordc.creatorZimmer, Ralf
Data Creatordc.creatorGhazal, Peter
Data Creatordc.creatorHaas, Jurgen
Date Accessioneddc.date.accessioned2016-08-02T16:13:00Z
Date Availabledc.date.available2016-08-02T16:13:00Z
Citationdc.identifier.citationGriffiths, Samantha; Koegl, Manfred; Boutell, Chris; Zenner, Helen; Crump, Colin; Pica, Francesca; Gonzalez, Orland; Friedel, Caroline; Barry, Gerald; Martin, Kim; Craigon, Marie; Chen, Rui; Kaza, Lakshmi; Fossum, Even; Fazakerley, John; Efstathiou, Stacey; Volpi, Antonio; Zimmer, Ralf; Ghazal, Peter; Haas, Jurgen. (2016). Small interfering RNAs (siRNA) genome-scale screen data for investigating host factors (HFs) involved in Herpes Simplex Virus Type 1 Replication, 2012-2013 [dataset]. University of Edinburgh. Medical school. Division of infection & Pathway Medicine. https://doi.org/10.7488/ds/1451.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2069
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/1451
Dataset Description (abstract)dc.description.abstractSmall interfering RNA (siRNA) data associated with the following manuscript by Griffiths et al.(2013): A Systematic Analysis of Host Factors Reveals a Med23-Interferon-l Regulatory Axis against Herpes Simplex Virus Type 1 Replication. Herpes simplex virus type 1 (HSV-1) is a neurotropic virus causing vesicular oral or genital skin lesions, meningitis and other diseases particularly harmful in immunocompromised individuals. To comprehensively investigate the complex interaction between HSV-1 and its host we combined two genome-scale screens for host factors (HFs) involved in virus replication. A yeast two-hybrid screen for protein interactions and a RNA interference (RNAi) screen with a druggable genome small interfering RNA (siRNA) library confirmed existing and identified novel HFs which functionally influence HSV-1 infection. Hela cells were reverse-transfected with siRNA SMARTpools (4 siRNAs per gene). After 48 h the siRNAs were tested for cytotoxicity (3 replicates) or the capacity to influence replication of the HSV-1 GFP reporter virus C12 (6 replicates) from 24 to 80 h post-infection. Virus replication slopes during the linear phase were calculated and normalized to mock-transfected cells. Replication slopes were then compared to replication upon knockdown of essential (ICP4, VP16) or non-essential (VP11/12) viral genes, a cellular receptor for HSV-1 (HVEM) or control RISC-free siRNA (RSCF).en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. Medical school. Division of infection & Pathway Medicineen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttp://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003514en_UK
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectSmall interfering RNAsen_UK
Subjectdc.subjectHost-pathogen interactionen_UK
Subjectdc.subjectViral replicationen_UK
Subjectdc.subjectprotein interactionsen_UK
Subjectdc.subjectInterferonsen_UK
Subjectdc.subjecttransfectionen_UK
Subjectdc.subjectRNA interferenceen_UK
Subjectdc.subjectHeLa cellsen_UK
Subject Classificationdc.subject.classificationSubjects allied to Medicineen_UK
Titledc.titleSmall interfering RNAs (siRNA) genome-scale screen data for investigating host factors (HFs) involved in Herpes Simplex Virus Type 1 Replicationen_UK
Typedc.typedataseten_UK

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