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Depositordc.contributorPilizota, Teuta
Depositordc.contributorWard, Paulineen
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Council
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Council
Data Creatordc.creatorPilizota, Teuta
Data Creatordc.creatorStevenson, Keiran
Data Creatordc.creatorClark, Ivan
Date Accessioneddc.date.accessioned2016-07-21T16:00:03Z
Date Availabledc.date.available2016-07-21T16:00:03Z
Citationdc.identifier.citationPilizota, Teuta; Stevenson, Keiran; Clark, Ivan. (2016). General calibration of microbial growth in microplate readers: Data supporting Figure 4, [dataset]. University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology. https://doi.org/10.7488/ds/1447.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2064
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/1447
Dataset Description (abstract)dc.description.abstractData supporting figure 4 of the paper (Stevenson et al, https://doi.org/10.1101/061861). Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from antibiotic efficacy studies, studies of growth under different nutritional or stress environments, to studies of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.
Dataset Description (TOC)dc.description.tableofcontentsData for the other figures are available in the same DataShare Collection.
Publisherdc.publisherUniversity of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology
Relation (Is Referenced By)dc.relation.isreferencedbyhttps://doi.org/10.1101/061861
Relation (Is Referenced By)dc.relation.isreferencedby"General calibration of microbial growth in microplate readers" Stevenson K, McVey AF, Clark IBN, Swain PS, Pilizota T bioRXiv doi: https://doi.org/10.1101/061861
Relation (Is Referenced By)dc.relation.isreferencedbyStevenson, K, McVey, A, Clark, IBN, Swain, P & Pilizota, T 2016, 'General calibration of microbial growth in microplate readers' Scientific Reports., DOI: 10.1038/srep38828
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectoptical density measurements
Subject Classificationdc.subject.classificationBiological Sciences
Titledc.titleGeneral calibration of microbial growth in microplate readers: Data supporting Figure 4
Typedc.typedataset

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