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Depositordc.contributorWest, John
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Data Creatordc.creatorDorà, Natalie J.
Data Creatordc.creatorWest, John D.
Data Creatordc.creatorHill, Robert E.
Data Creatordc.creatorCollinson, J. Martin
Date Accessioneddc.date.accessioned2016-02-11T16:31:41Z
Date Availabledc.date.available2016-02-11T16:31:41Z
Citationdc.identifier.citationDorà, Natalie J.; Hill, Robert E.; Collinson, J. Martin; West, John D. (2016). Lineage tracing in the adult mouse corneal epithelium supports the limbal epithelial stem cell hypothesis with intermittent periods of stem cell quiescence, [dataset]. University of Edinburgh Medical School. https://doi.org/10.7488/ds/1341.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/1921
Persistent Identifierdc.identifier.urihttps://doi.org/10.7488/ds/1341
Dataset Description (abstract)dc.description.abstractThe limbal epithelial stem cell (LESC) hypothesis proposes that LESCs in the corneal limbus maintain the corneal epithelium both during normal homeostasis and wound repair. The alternative corneal epithelial stem cell (CESC) hypothesis proposes that LESCs are only involved in wound repair and CESCs in the corneal epithelium itself maintain the corneal epithelium during normal homeostasis. We used tamoxifen-inducible, CreER-loxP lineage tracing to distinguish between these hypotheses. Clones of labelled cells were induced in adult CAGG-CreER;R26R-LacZ reporter mice and their distributions analysed after different chase periods. Short-lived clones, derived from labelled transient amplifying cells, were shed during the chase period and long-lived clones, derived from stem cells, expanded. At 6 weeks, labelled clones appeared at the periphery, extended centripetally as radial stripes and a few reached the centre by 14 weeks. Stripe numbers depended on the age of tamoxifen treatment. Stripes varied in length, some were discontinuous, few reached the centre and almost half had one end at the limbus. Similar stripes extended across the cornea in CAGG-CreER;R26R-mT/mG reporter mice. The distributions of labelled clones are inconsistent with the CESC hypothesis and support the LESC hypothesis if LESCs cycle between phases of activity and quiescence, each lasting several weeks.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh Medical Schoolen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttp://www.ncbi.nlm.nih.gov/pubmed/26554513en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyDorà NJ, Hill RE, Collinson JM, West JD (2015) "Lineage tracing in the adult mouse corneal epithelium supports the limbal epithelial stem cell hypothesis with intermittent periods of stem cell quiescence". Stem Cell Res. 2015 Nov;15(3):665-77. doi: 10.1016/j.scr.2015.10.016. Epub 2015 Oct 28.en
Rightsdc.rightsCreative Commons Attribution 4.0 International Public Licenseen
Subjectdc.subjectcorneaen_UK
Subjectdc.subjectlimbal epithelial stem cells
Subjectdc.subjectlineage tracing
Subjectdc.subjectstem cell quiescence
Subjectdc.subjectstem cells
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleLineage tracing in the adult mouse corneal epithelium supports the limbal epithelial stem cell hypothesis with intermittent periods of stem cell quiescenceen_UK
Alternative Titledc.title.alternativeLineage tracing in the adult mouse corneal epithelium_Dora et al_DataShareen_UK
Typedc.typedataseten_UK

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