Rosser, Susan; Kleinjan, Dirk-Jan. (2017). Data in support of 'Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors', [dataset]. University of Edinburgh, UK Centre for Mammalian Synthetic Biology, School of Biological Sciences.
This dataset contains a file with images of the full Western blots used in the manuscript "Drug-tunable multidimensional Synthetic Gene Control using Inducible Degron-tagged dCas9 Effectors", plus a file with DNA sequences of constructs used in the study.
The nuclease-deactivated variant of CRISPR/Cas9 proteins (dCas9) fused to heterologous transactivation domains can act as a potent guide RNA sequence-directed inducer or repressor of gene expression in mammalian cells. In such a system the long-term presence of a stable dCas9 effector can be a draw-back precluding the ability to switch rapidly between repressed and activated target gene expression states, imposing a static environment on the synthetic regulatory circuits in the cell. To address this issue we have generated a toolkit of conditionally degradable or stabilisable orthologous dCas9/Cpf1 effector proteins, thus opening options for multidimensional control of functional activities through combinations of orthogonal, drug-tunable artificial transcription factors.