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Depositordc.contributorHulme, Alison
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherCRUK - Cancer Research UKen_UK
Funderdc.contributor.otherERC - European Research Councilen_UK
Time Perioddc.coverage.temporalstart=2014-09-01; end=2017-03-31; scheme=W3C-DTFen
Data Creatordc.creatorTipping, William J
Data Creatordc.creatorLee, Martin
Data Creatordc.creatorSerrels, Alan
Data Creatordc.creatorBrunton, Valerie G
Data Creatordc.creatorHulme, Alison N
Date Accessioneddc.date.accessioned2017-05-18T11:33:52Z
Date Availabledc.date.available2017-05-18T11:33:52Z
Citationdc.identifier.citationTipping, William J; Lee, Martin; Serrels, Alan; Brunton, Valerie G; Hulme, Alison N. (2017). Imaging Drug Uptake by Bioorthogonal Stimulated Raman Scattering Microscopy, 2014-2017 [dataset]. University of Edinburgh, School of Chemistry. http://dx.doi.org/10.7488/ds/2046.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2711
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/2046
Dataset Description (abstract)dc.description.abstractStimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficay of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal photoxicity and low photobleaching associated with SRS microscopy has enabled real time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh, School of Chemistryen_UK
Subjectdc.subjectbioorthogonal imagingen_UK
Subjectdc.subjectStimulated Raman Scattering (SRS)en_UK
Subjectdc.subjectbisalkyneen_UK
Subjectdc.subjectanisomycinen_UK
Subjectdc.subjectcell cycle markersen_UK
Subjectdc.subject5-ethynyl-2'-deoxyuridine (EdU)en_UK
Subject Classificationdc.subject.classificationPhysical Sciences::Medicinal Chemistryen_UK
Titledc.titleImaging Drug Uptake by Bioorthogonal Stimulated Raman Scattering Microscopyen_UK
Typedc.typedataseten_UK

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