Show simple item record

Depositordc.contributorKrstajic, Nikola
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Data Creatordc.creatorKufcsak, Andras
Data Creatordc.creatorKrstajic, Nikola
Date Accessioneddc.date.accessioned2017-04-18T08:22:50Z
Date Availabledc.date.available2017-04-24
Citationdc.identifier.citationKufcsak, Andras; Krstajic, Nikola. (2017). Datasets for Kufcsak et al Optics Express paper "Time-resolved spectroscopy at 19,000 lines per second using a CMOS SPAD line array enables advanced biophotonics applications", [dataset]. University of Edinburgh. School of Engineering. http://dx.doi.org/10.7488/ds/2000.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2659
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/2000
Dataset Description (abstract)dc.description.abstractA SPAD-based line sensor fabricated in 130 nm CMOS technology capable of acquiring time-resolved fluorescence spectra (TRFS) in 8.3 milliseconds is presented. To the best of our knowledge, this is the fastest time correlated single photon counting (TCSPC) TRFS acquisition reported to date. The line sensor is an upgrade to our prior work and incorporates: i) parallelized interface from sensor to surrounding circuitry enabling high line rate to the PC (19,000 lines/s) and ii) novel time-gating architecture where detected photons in the OFF region are rejected digitally after the output stage of the SPAD. The time-gating architecture was chosen to avoid electrical transients on the SPAD high voltage supplies when gating is achieved by excess bias modulation. The time-gate has an adjustable location and time window width allowing the user to focus on time-events of interest. On-chip integrated center-of-mass (CMM) calculations provide efficient acquisition of photon arrivals and direct lifetime estimation of fluorescent decays. Furthermore, any of the SPC, TCSPC and on-chip CMM modes can be used in conjunction with the time-gating. The higher readout rate and versatile architecture greatly empower the user and will allow widespread applications across many techniques and disciplines. Here we focused on 3 examples of TRFS and time-gated Raman spectroscopy: i) kinetics of chlorophyll A fluorescence from an intact leaf; ii) kinetics of a thrombin biosensor FRET probe from quenched to fluorescence states; iii) ex vivo mouse lung tissue autofluorescence TRFS; iv) time-gated Raman spectroscopy of toluene at 3056 cm-1 peak. To the best of our knowledge, we detect spectrally for the first time the fast rise in fluorescence lifetime of chlorophyll A in a measurement over single fluorescent transient.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Engineeringen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttp://www.research.ed.ac.uk/portal/en/publications/timeresolved-spectroscopy-at-19000-lines-per-second-using-a-cmos-spad-line-array-enables-advanced-biophotonics-applications(8dbb3f83-9ab7-40b3-9388-72e5d5385025).htmlen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyKufcsak, A, Erdogan, A, Walker, R, Katjana, E, Tanner, MG, Megia-Fernandez, A, Scholefield, E, Emanuel, P, Dhaliwal, K, Bradley, M, Henderson, R & Krstajic, N 2017, 'Time-resolved spectroscopy at 19,000 lines per second using a CMOS SPAD line array enables advanced biophotonics applications' Optics Express.
Subjectdc.subjecttime resolved spectroscopyen_UK
Subjectdc.subjectCMOS SPAD line arrayen_UK
Subjectdc.subjectFluorescence spectroscopyen_UK
Subject Classificationdc.subject.classificationPhysical Sciences::Optical Physicsen_UK
Titledc.titleDatasets for Kufcsak et al Optics Express paper "Time-resolved spectroscopy at 19,000 lines per second using a CMOS SPAD line array enables advanced biophotonics applications"en_UK
Typedc.typedataseten_UK

Download All

Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record