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Depositordc.contributorPilizota, Teuta
Funderdc.contributor.otherBBSRC - Biotechnology and Biological Sciences Research Councilen_UK
Funderdc.contributor.otherEPSRC - Engineering and Physical Sciences Research Councilen_UK
Data Creatordc.creatorPilizota, Teuta
Data Creatordc.creatorStevenson, Keiran
Data Creatordc.creatorClark, Ivan
Date Accessioneddc.date.accessioned2017-03-01T15:46:51Z
Date Availabledc.date.available2017-03-01T15:46:51Z
Citationdc.identifier.citationPilizota, Teuta; Stevenson, Keiran; Clark, Ivan. (2017). General calibration of microbial growth in microplate readers: Data supporting Figures 1-3, [dataset]. University of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biology. http://dx.doi.org/10.7488/ds/1980.en
Persistent Identifierdc.identifier.urihttp://hdl.handle.net/10283/2634
Persistent Identifierdc.identifier.urihttp://dx.doi.org/10.7488/ds/1980
Dataset Description (abstract)dc.description.abstractData supporting figures 1-3 of the paper (Stevenson et al, http://dx.doi.org/10.1101/061861). Optical density (OD) measurements of microbial growth are one of the most common techniques used in microbiology, with applications ranging from antibiotic efficacy studies, studies of growth under different nutritional or stress environments, to studies of different mutant strains, including those harbouring synthetic circuits. OD measurements are performed under the assumption that the OD value obtained is proportional to the cell number, i.e. the concentration of the sample. However, the assumption holds true in a limited range of conditions and calibration techniques that determine that range are currently missing. Here we present a set of calibration procedures and considerations that are necessary to successfully estimate the cell concentration from OD measurements.en_UK
Dataset Description (TOC)dc.description.tableofcontentsData for the other figures are available in the same DataShare Collection - see http://datashare.is.ed.ac.uk/handle/10283/2058. N.B. the file "OD Paper data-Fig1-3.zip" is identical to the file of the same name in the earlier version of this data deposit at http://dx.doi.org/10.7488/ds/1446.en_UK
Languagedc.language.isoengen_UK
Publisherdc.publisherUniversity of Edinburgh. School of Biological Sciences. Centre for Synthetic and Systems Biologyen_UK
Relation (Is Referenced By)dc.relation.isreferencedbyhttp://dx.doi.org/10.1038/srep38828en_UK
Relation (Is Referenced By)dc.relation.isreferencedbyStevenson, K, McVey, A, Clark, IBN, Swain, P & Pilizota, T 2016, 'General calibration of microbial growth in microplate readers' Scientific Reports., DOI: 10.1038/srep38828en_UK
Supersedesdc.relation.replaceshttp://dx.doi.org/10.7488/ds/1446en_UK
Subjectdc.subjectoptical density measurementsen_UK
Subject Classificationdc.subject.classificationBiological Sciencesen_UK
Titledc.titleGeneral calibration of microbial growth in microplate readers: Data supporting Figures 1-3en_UK
Typedc.typedataseten_UK


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