Johnson, Craig; Clements, Dylan. (2017). Stimulation of canine joint cells to model osteoarthritis, [dataset]. Roslin Institute, University of Edinburgh. http://dx.doi.org/10.7488/ds/1458.
Canine chondrocytes and osteoblasts were embedded in an agarose matrix and simultaneously stimulated with cytokines whilst being cyclically compressed. Both cell types were compressed for 4 hours at 1 Hz followed by a 24 hour recovery incubation.
The chondrocytes were stimulated with 5 ng/mL of IL8 for the duration of compression, but removed for the recovery phase.
Osteoblasts were stimulated with 0.5 ng/mL IL8 and 0.5 ng/mL IL1b for the duration of the compression and for the entirety of the 24 hour incubation step.